Kinetics of Photoinduced RNA Refolding by Real‐Time NMR Spectroscopy

Abstract

By introducing a photolabile group in the Watson–Crick base-pairing site of a guanosine residue, a bistable 20-base RNA sequence was forced into a less stable conformation. A single laser pulse released the native sequence, and the subsequent refolding equilibration was monitored with time-resolved NMR spectroscopy (see spectra). This permitted a quantitative description of the refolding process. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2005/z462724_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.