Concepedia

Identification and validation of housekeeping genes as internal control for gene expression in an intravenous LPS inflammation model in chickens

S. De Boever, Carl Vangestel, Julie De Backer, Siska Croubels, Stanislas U. Sys

Veterinary Immunology and Immunopathology · 2008 · 157 citations · 17 references

DOI

Concepts

References

17

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Jo Vandesompele, Katleen De Preter, Filip Pattyn et al. · Genome biology · 2002 · 20.1K citations · Full text

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Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations

Michael W. Pfaffl, Aleš Tichopád, Christian Prgomet et al. · Biotechnology Letters · 2004 · 5.1K citations

, , +12

Housekeeping genes as internal standards: use and limits

Olivier Thellin, Willy Zorzi, Bernard Lakaye et al. · Journal of Biotechnology · 1999 · 1.6K citations · Full text

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Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR

Thomas D. Schmittgen, Brian A. Zakrajsek · Journal of Biochemical and Biophysical Methods · 2000 · 1.2K citations

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Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction.

Stephen A. Bustin, Tania Nolan · PubMed · 2004 · 1.1K citations

, , +13