Selective Medium for Isolating<i>Lasiodiplodia theobromae</i>

Abstract

Six chemicals were tested in vitro for their effect on the growth of Lasiodiplodia theobromae. Three were selected for further evaluation on 14 of the fastest growing fungi commonly associated with L. theobromae on Pinus elliottii seeds. Tannic acid suppressed Rhizopus sp., Drechslera sp., Trichoderma sp., and Sphaeropsis sapinea, four of the fastest growing test fungi. Benodanil and Tridemorph suppressed most of the other fungi. The selective medium, consisting of 33.6 g/L of malt extract agar (MEA), 3,000 μg/ml of tannic acid, 50 μg/ml of benodanil, and 0.5 μg/ml of tridemorph, was effective in suppressing all fungi selected for testing except L. theobromae