HPLC DETERMINATION OF PESTICIDE RESIDUE ISOLATED FROM FOOD MATRICES

Abstract

ABSTRACT Methods for the extraction, separation, detection, and quantification of ten herbicide, insecticide, and fungicide residues in fruits, vegetables, and cereal were evaluated. The extraction of the residues was achieved using liquid–liquid extraction (LLE), solid-phase extraction (SPE), and matrix solid-phase dispersion (MSPD). Determination was carried out by reversed-phase high performance liquid chromatography (RP-HPLC) with column switching and diode array detection (DAD). Recoveries, at spiked concentrations below the maximum acceptable residue levels established by the Polish Government, were between 68.7 and 105.0% with relative standard deviations ranging from 0.5 to 6.2%. The limits of quantification varied from 0.02 to 0.25 μg/g.