The Glucoamylase-Encoding Gene (<i>glaB</i>) Is Expressed in Solid-State Culture with A Low Water Content

Abstract

Solid-state culture encourages high-level enzyme secretion by Aspergillus oryzae. Using the real-time quantitative reverse transcriptase-polymerase chain reaction, we confirmed that expression of the glucoamylase-encoding gene in A. oryzae cultured in solid-state culture depends on the water content of the culture.