Electronic Detection of Lectins Using Carbohydrate-Functionalized Nanostructures: Graphene <i>versus</i> Carbon Nanotubes

Abstract

Here we investigated the interactions between lectins and carbohydrates using field-effect transistor (FET) devices comprised of chemically converted graphene (CCG) and single-walled carbon nanotubes (SWNTs). Pyrene- and porphyrin-based glycoconjugates were functionalized noncovalently on the surface of CCG-FET and SWNT-FET devices, which were then treated with 2 μM nonspecific and specific lectins. In particular, three different lectins (PA-IL, PA-IIL, and ConA) and three carbohydrate epitopes (galactose, fucose, and mannose) were tested. The responses of 36 different devices were compared and rationalized using computer-aided models of carbon nanostructure/glycoconjugate interactions. Glycoconjugate surface coverage in addition to one-dimensional structures of SWNTs resulted in optimal lectin detection. Additionally, lectin titration data of SWNT- and CCG-based biosensors were used to calculate lectin dissociation constants (K(d)) and compare them to the values obtained from the isothermal titration microcalorimetry technique.