Construction of an attenuated Pseudomonas fluorescens strain and evaluation of its potential as a cross-protective vaccine

Molecular cloning: A laboratory manual

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Site-directed mutagenesis by overlap extension using the polymerase chain reaction

Steffan N. Ho, Henry D. Hunt, Robert M. Horton et al. · Gene · 1989 · 7.5K citations

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A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR

Virginia L. Miller, John J. Mekalanos · Journal of Bacteriology · 1988 · 2.1K citations · Full text

Life Sciences, Genetics, Molecular Biology +17

Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization

Andrew J. Link, Devan Phillips, George M. Church · Journal of Bacteriology · 1997 · 875 citations

Life Sciences, Genetics, Molecular Biology +15

Regulation of ferric iron transport in Escherichia coli K12: Isolation of a constitutive mutant

Klaus Hantke · Molecular and General Genetics MGG · 1981 · 557 citations

Life Sciences, Biochemistry, Microbiology +14