Antibody scFv fragments without disulfide bonds, made by molecular evolution

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

F. William Studier, Barbara A. Moffatt · Journal of Molecular Biology · 1986 · 6.4K citations

Life Sciences, Microbiology, Molecular Biology +9

Sequences of proteins of immunological interest

Analytical Biochemistry · 1984 · 3.4K citations

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High efficiency transformation of Escherichia coli with plasmids

Hiroaki Inoue, Hiroshi Nojima, Hiroto Okayama · Gene · 1990 · 2.3K citations

Life Sciences, Biotechnology, Microbiology +13

Rapid evolution of a protein in vitro by DNA shuffling

Willem P.C. Stemmer · Nature · 1994 · 1.9K citations

Life Sciences, Genetics, Molecular Biology +10

An hsp70-like protein in the ER: Identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein

Sean Munro, Hugh R.B. Pelham · Cell · 1986 · 1.5K citations

Life Sciences, Biochemistry, Molecular Biology +10