Cloning and disruption of the b-isopropylmalate dehydrogenase gene ( LEU2  ) of Pichia stipitis with URA3 and recovery of the double auxotroph

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.3K citations · Full text

Life Sciences, Microbiology, Molecular Biology +10

A comprehensive set of sequence analysis programs for the VAX

John Devereux, Paul Haeberli, Oliver Smithies · Nucleic Acids Research · 1984 · 14.4K citations · Full text

Life Sciences, Bioinformatics, Genetics +8

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

Jef D. Boeke, Francois La Croute, Gerald R. Fink · Molecular and General Genetics MGG · 1984 · 2.4K citations

Life Sciences, Genetics, Molecular Biology +8

Progressive sequence alignment as a prerequisitetto correct phylogenetic trees

Russell F. Doolittle · Journal of Molecular Evolution · 1987 · 1.9K citations

Life Sciences, Bioinformatics, Evolutionary Biology +14

A Method for Gene Disruption That Allows Repeated Use of URA3 Selection in the Construction of Multiply Disrupted Yeast Strains

Eric Alani, Lei Cao, Nancy Kleckner · Genetics · 1987 · 883 citations

Life Sciences, Genetics, Molecular Genetics +15