Survival of legionella pneumophila and salmonella typhimurium in biofilm systems

Abstract

Research Article| June 01 1997 Survival of legionella pneumophila and salmonella typhimurium in biofilm systems R. Armon; R. Armon *Environmental and Water Resources Engineering, Technion, Haifa 32000, Israel Search for other works by this author on: This Site PubMed Google Scholar J. Starosvetzky; J. Starosvetzky *Environmental and Water Resources Engineering, Technion, Haifa 32000, Israel Search for other works by this author on: This Site PubMed Google Scholar T. Arbel; T. Arbel *Environmental and Water Resources Engineering, Technion, Haifa 32000, Israel Search for other works by this author on: This Site PubMed Google Scholar M. Green M. Green **Faculty of Agricultural Engineering, Technion, Haifa 32000, Israel Search for other works by this author on: This Site PubMed Google Scholar Water Sci Technol (1997) 35 (11-12): 293–300. https://doi.org/10.2166/wst.1997.0749 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Share Icon Share Facebook Twitter LinkedIn Email Tools Icon Tools Cite Icon Cite Permissions Search Site Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsThis Journal Search Advanced Search Citation R. Armon, J. Starosvetzky, T. Arbel, M. Green; Survival of legionella pneumophila and salmonella typhimurium in biofilm systems. Water Sci Technol 1 June 1997; 35 (11-12): 293–300. doi: https://doi.org/10.2166/wst.1997.0749 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex Biofilms are found in many water supply systems where they form an environment in which different bacteria can be entrapped for long periods. Besides the aesthetic aspect, biofilm has a major contribution in biocorrosion, disinfection inefficiency and possibly may act as a reservoir for pathogenic and non-pathogenic microorganisms. In the present study, two pathogenic bacteria Legionella pneumophila and Salmonella typhimurium WG-49 were introduced into a biofilm simulation flow system supplied with sterile and non-sterile tap water. The survival of these microorganisms into the biofilm formed on glass and PVC coupons at two temperatures (24°C and 36°C) was compared in this system. On glass supports, under sterile conditions at 36°C, Legionella pneumophila sg3 decreased by 6 logs during 40d continuous recirculation. Under non-sterile conditions, L. pneumophila decreased by only half log <48d. S. typhimurium WG-49 under the same conditions showed an increase of 3 logs in the sterile system for 31d, while in the non-sterile system it dropped by only 0.5 log for 20d. At 24°C, L. pneumophila remained stable for >40d under sterile conditions. In non-sterile conditions, L. pneumophila dropped by 1 log for 35d. S. typhimurium, in a sterile system, remained almost unchanged, while in the non-sterile system an increase of 3 logs was observed for the first 21d and thereafter a decrease of 2 logs for the next 21d of the experiment. L. pneumophila on PVC coupons at 36°C survived better compared with glass support. The experimental data show that survival of pathogenic microorganisms into biofilm is variable and depends on many factors, making the survival prediction a difficult task. However, the survival results of L. pneumophila and S. typhimurium in time terms should raise important questions on their potential threat in water distribution systems. Biofilm, Salmonella, Legionella, survival, sterile/non-sterile, tap water, glass, PVC This content is only available as a PDF. © IWA Publishing 1997 You do not currently have access to this content.