Isolation of the human cdk2 gene that encodes the cyclin A- and adenovirus E1A-associated p33 kinase

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.3K citations · Full text

Life Sciences, Microbiology, Molecular Biology +10

Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia

Randall K. Saiki, Stephen J. Scharf, Fred Faloona et al. · Science · 1985 · 9K citations

Medicine, Genetics, Molecular Genetics +17

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

Donald B. Smith, Kevin S. Johnson · Gene · 1988 · 6K citations

Life Sciences, Biochemistry, Molecular Biology +12

Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

Don W. Cleveland, S G Fischer, Marc W. Kirschner et al. · Journal of Biological Chemistry · 1977 · 5.6K citations · Full text

Life Sciences, Biochemistry, Molecular Biology +17

Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5

Frank L. Graham, W. C. Russell, James R. Smiley et al. · Journal of General Virology · 1977 · 4.9K citations

Life Sciences, Molecular Biology, Molecular Genetics +16