Effect of sulforaphane on cell growth, G0/G1 phase cell progression and apoptosis in human bladder cancer T24 cells

Abstract

Isothiocyanates (ITCs) from cruciferous vegetables have been shown to be effective in blocking initiation as well as progression of a range of chemically-induced tumors in animal models. In this study, sulforaphane, the most extensively studied ITC, was found to suppress the growth of T24 bladder cancer cells in vitro in a dose-dependent manner. Sulforaphane inhibited the proliferation of T24 cells with IC(5)0 values 26.9 and 15.9 microM following 24 and 48 h treatments. Sulforaphane (5-20 microM) induced early apoptosis and blocked cell cycle progression at G(0)/G(1) phase which was associated with upregulation of cyclin-dependent kinase inhibitor p27 expression. These results support a role for sulforaphane as an effective agent in the chemoprevention of bladder cancer.