Acta Crystallographica Section D Biological Crystallography · 2003 · 11 citations · 11 references
Natural SciencesBiochemistryChemistryCrystal StructureGlycobiologyStructural BiologyCrystallization AnalysisCrystallization ProcessesCrystallographyStructure DeterminationX-ray CrystallographyX-ray DiffractionCrystal Structure DeterminationCrystal Structure RefinementCrystallographic CharacterizationElectron DensityUnit Cell ParametersX-ray Crystallographic RefinementX-ray Diffraction AnalysisX-ray Structure Determination
A fucose-specific lectin from Aleuria aurantia was crystallized in its native form and was also cocrystallized with HgCl(2). Crystallization was performed using the sitting-drop vapour-diffusion method with ammonium sulfate as a precipitant. Both the Hg-free native crystals and the Hg cocrystals belong to the hexagonal space group P6(5)22. The unit-cell parameters for the Hg-free form are a = 84.0, c = 250.1 A and for the Hg cocrystals are a = 83.9, c = 254.3 A. Both forms of the crystals diffract X-rays to 2.3 A resolution and are suitable for high-resolution crystal structure determination. Initial phasing was successfully performed by the MAD method using the Hg cocrystals and the electron density obtained was good enough for model building.
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Solvent content of protein crystals
Brian W. Matthews · Journal of Molecular Biology · 1968 · 8K citations