Characterization of trypsinogens 1 and 2 in two human pancreatic adenocarcinoma cell lines; CFPAC‐1 and CAPAN‐1

Abstract

Proteins with trypsin‐like immunoreactivity (first detected by a specific immunoenzymatic assay) were isolated from CAPAN‐1 and CFPAC‐1 cell culture‐conditioned media by chromatography on an immunoadsorbent prepared with a polyclonal antibody directed against trypsin 1. The adsorbed proteins were devoid of free trypsin activity but trypsin activity was present after enterokinase activation demonstrating that the immunoreactive trypsin present in cell supernatants corresponds to trypsinogens. When characterised by Western blotting using a monoclonal antibody directed against human trypsin 1 two protein bands corresponding to trypsinogen 1 (23 kDa) and trypsinogen 2 (25 kDa) gave a positive reaction. These results demonstrate the presence of trypsinogens 1 and 2 in CAPAN‐1 and CFPAC‐1 cells and in their culture‐conditioned media.