Induction of Giant Cells in Suspension Cultures of Arachis hypogaea, L. by Massive Irradiation

Abstract

The growth of V-56R peanut (Arachis hypogaea, L.) cell-suspension cultures was inhibited as a function of dose (5-500 krad) of radiation, however, protein synthesis showed an immediate response only after a dose of more than 50 krad. Morphological observations made on the irradiated cells indicated that while cell division is completely arrested after a dose of 50-500 krad, cell expansion seems to be least affected and 50-60% cells become large filamentous giant cells. The frequency of giant cells increased during postirradiation period of 4-5 weeks. Radiation-induced giant cells showed an active cyclosis, denser cytoplasm and higher number of starch grains. Protein synthesis continued even after a massive dose (500 krad) of radiation at approximately 45-55% of controls, if the cells were incubated in fresh medium after irradiation, and showed an apparent recovery during postirradiation period. The effect of radiation on protein synthesis was also dependent on the age of the cultures and presence of condi...