A single amino acid can determine the DNA binding specificity of homeodomain proteins

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.1K citations · Full text

Dna, Engineering, Dna Analysis +20

Rapid and efficient site-specific mutagenesis without phenotypic selection.

Thomas A. Kunkel · Proceedings of the National Academy of Sciences · 1985 · 7.3K citations · Full text

Engineering, Reverse Genetics, Efficient Site-specific Mutagenesis +14

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

F. William Studier, Barbara A. Moffatt · Journal of Molecular Biology · 1986 · 6.4K citations

Selective High-level Expression, Natural Sciences, Phage Biology +8

Mutations affecting segment number and polarity in Drosophila

Christiane Nüsslein‐Volhard, Eric Wieschaus · Nature · 1980 · 4.4K citations

Population Genetics, Developmental Genetics, Genetics +6

A conserved DNA sequence in homoeotic genes of the Drosophila Antennapedia and bithorax complexes

William McGinnis, Michael Levine, Ernst Hafen et al. · Nature · 1984 · 1.1K citations

Biology, Natural Sciences, Genetics +11