Canadian Journal of Botany · 1973 · 56 citations · 0 references
Life SciencesPlant BiologyPlant SciencesCellular MetabolismPlant Cell BiologyPlant NutritionNitrogen AssimilationNitrogen CycleNitrogen ManagementNitrogen MetabolismNitrogen NutritionNutrient AssimilationBiomass ProductionCell Culture PerformanceInorganic Growing MediaNutrient Medium OptimizationPlant Biomass ProductionPlant Cell Suspension CulturePlant-available NitrogenSuspension Cell Culture
A chemically defined 71V medium was designed in accordance with data from studies of NH 4 + and NO 3 − requirements for biomass production of carrot cell suspension culture (CA-68 line). Under the described conditions, the optimum ratio of NH 4 + :NO 3 − was 20:80, and the optimum total nitrogen level was about 500 mg N per liter of medium. The observed changes in pH of the different inorganic media during the growth of the culture might be an important factor influencing the nitrogen metabolism of the cells.