Performance of affinity chromatography columns

Abstract

Abstract Performance of affinity chromatography columns was studied by measuring the rates of adsorption and elution of trypsin in a Sepharose 4B‐soybean trypsin inhibitor column and a Sepharose 4B‐arginine peptides column. The volumetric coefficient for trypsin transfer was evaluated from the break‐through curves of trypsin, and elution profiles bed height of Sepharose 4B‐STI column was estimated based on these results.