Bioscience Biotechnology and Biochemistry · 2006 · 41 citations · 17 references
Life SciencesBiotechnologyMicrobiologyGenetic EngineeringMicrobial GeneticsSynthetic BiologyGenetic Engineering Of MicroorganismsMicrobial Metabolic EngineeringMicrobial Synthetic BiologyPlasmid EngineeringPlasmid GeneticsStrain EngineeringBacillus SubtilisGenetic Engineering Of MicrobesGlutamate SynthesisGlutamic Acid FermentationL-glutamate FermentationMicrobial Strain EngineeringSynthetic Biology For FermentationSynthetic Plasmid Construction
The pgsBCA-gene disruptant from Bacillus subtilis ISW1214, i.e., MA41, does not produce poly-gamma-glutamate (PGA). We newly constructed an MA41 recombinant bearing the plasmid-borne PGA synthetic system, in which PGA production was strictly controlled by the use of xylose. Unlike the parent strain, ISW1214, the genetically engineered strain produced abundant PGA in both L-glutamate-rich and D-glutamate-rich media.
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Nicola R. Stanley‐Wall, Beth Lazazzera · Molecular Microbiology · 2005 · 259 citations
Makoto Ashiuchi, Kenji Soda, Haruo Misono · Biochemical and Biophysical Research Communications · 1999 · 180 citations