COMPONENTS OF FAST AND SLOW PHASES OF AXOPLASMIC FLOW¹

Abstract

Abstract— Cat ventral roots removed at times from hours to days after injection of [ 3 H]leucine into the seventh lumbar and first sacral spinal cord segments were homogenized and subjected to sub‐cellular fractionation. The soluble fraction was further analysed for protein content by column chromatography with Sephadex. The fast phase of axoplasmic flow carries down labelled free leucine, polypeptide, and soluble protein. The slow phase of axoplasmic flow carries down labelled soluble protein. The sub‐cellular components were labelled with the small particulate fraction showing a higher specific activity than the other sub‐cellular fractions.