ACRYLAMIDE FLUORESCENCE QUENCHING APPLIED TO TYROSYL RESIDUES IN PROTEINS

Abstract

Abstract— The quenching of the tyrosyl emission of proteins which lack tryptophanyl residues was investigated with the neutral quencher, acrylamide. The screening effect of acrylamide at the excitation wavelength, considered until now, as an obstacle to its use in tyrosine quenching studies, was allowed for by means of a theoretical correction. The value of this method was demonstrated by a comparative study using cesium ions as quenchers, which gave similar results on fluorophore location in the protein.