Angewandte Chemie International Edition · 2001 · 144 citations · 14 references
Natural SciencesBiochemistryMolecular BiologyBioorthogonal ChemistryBiophysical ChemistryBase PairingOligonucleotide ChemistryPhotocatalysisPhotochemical SynthesisPhotochemistryAzobenzene PhotochromismChemical Modification Of OligonucleotidesOligonucleotide HybridizationOligonucleotide SynthesisOligonucleotide ThermodynamicsPhotocleavage Of Nucleic AcidsVisible Light Effects
A drop in melting point of 21.5°C is induced by the UV-photolytic trans→cis isomerization of the duplex formed between an oligonucleotide bearing two D-threoninol-tethered azobenzene moieties in the side chain and its complementary counterpart. On irradiation with visible light, the dissociated single-stranded oligonucleotides regenerate the duplex.
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