Applied and Environmental Microbiology · 2008 · 141 citations · 12 references
Life SciencesEnvironmental SciencesMicrobiologyEnvironmental MicrobiologyMicrobial EcologyWastewater TreatmentAnaerobic BacteriologyAnaerobic DigestionBiological Treatment ProcessesNucleic Acid ExtractionWastewater BiotreatmentWastewater MicrobiologyAnaerobic Sludge DigestionDna ExtractionEnvironmental Dna AnalysisEnvironmental Dna DetectionEnvironmental Dna ExtractionMicrobial Nucleic Acid ExtractionMicrobiological DiagnosticsWastewater Anaerobic Treatment
Recently, new DNA extraction techniques (using ethidium monoazide and propidium monoazide) have been developed to discriminate between alive and dead bacterial cells. Nevertheless, for complex environmental samples, no data are available yet. In the present study, these new methods were applied to anaerobic-fermentor sludge and the results were compared to a conventional microbiological approach.
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Andreas Nocker, Ching-Ying Cheung, Anne K. Camper · Journal of Microbiological Methods · 2006 · 1.1K citations