Canadian Journal of Chemistry · 1973 · 58 citations · 0 references
Natural SciencesBiochemistryChemistryAnalytical ChemistryCarbohydrate ChemistrySpectroscopyCarbohydrate AnalysisCarbohydrate CharacterizationCarbohydrate StructureCircular Dichroism SpectroscopyLiquid ChromatographySugar AnalysisAbsolute Configuration Of SugarsCarbohydrate Stereochemistry DeterminationCircular DichroismCircular Dichroism AnalysisCircular Dichroism Spectroscopy AnalysisStereochemical Analysis Of Sugars
The configuration (D or L) of a sugar may be determined conveniently by circular dichroism measurements at 213 nm on alditol acetates, or their methylated derivatives, where the acetoxy group acts as a chromophore. Only milligram quantities of material are required and the method is well suited to analyzing fractions obtained by gas–liquid chromatography. Structural information which may be derived from the c.d. spectra is briefly discussed.