Flow rate calibration. III. The use of stabilized biostandards to calibrate the flow rate and calculate absolute CD4⁺ T‐cell counts

Abstract

This study demonstrates that stabilized whole blood can be used for FRB. It has the advantage of being a full process control, in addition to costing less than latex beads with highly comparable results. As bench top flow cytometers are extremely stable, this is a low cost and robust alternative to bead based methods for generating absolute CD4 counts.