Identification of DNA Binding Motifs of the Mycobacterium tuberculosis PhoP/PhoR Two-Component Signal Transduction System

Abstract

A consensus sequence derived from the analysis of PhoP binding to four gene promoter regions is proposed. We show for the first time the involvement of a third direct repeat motif in this binding reaction. The consensus sequence was instrumented to study the global regulation mediated by PhoP in M. tuberculosis. This analysis leads to the identification of several genes that are potentially regulated by this key player.