The Mixed Agglutination Test in Studies on Human Transplantation

Abstract

Mixed agglutination with cell cultures was originally described by Högman¹and by Fagraeus and Espmark.²In this laboratory, application of this procedure for various research purposes^3-10was explored. One promising line seemed to be the detection of humoral antibodies accompanying homograft rejection in mice, rats, and rabbits.^7-10 In the present paper, preliminary studies on the demonstration of antibodies appearing as a result of homograft rejection in humans, as well as studies on human tissue typing will be reported. <h3>Materials and Methods</h3><h3>Human Cell Lines.—</h3> HeLa,¹¹AV-3,¹²WI-38,¹³HEp-2,¹⁴D-6,¹⁵and KB¹⁶cells were cultured as described previously^3,4and studied when a whole monolayer was developed, usually after seven days of incubation. <i>Primary cell cultures</i>were set up from human tonsils. The tissues were obtained from Children's Hospital, Buffalo, immediately after tonsillectomy. In preparing cell cultures, previously described methods^3,4