Culture characteristics of four permanent lines of human cancer cells.

Abstract

Summary The cultural and morphological characteristics of three strains of human epidermoid cancer cells (H.Ep. #1, H.Ep. #2, and H.Ep. #3) and one human embryonal rhabdomyosarcoma (H.Emb.Rh. #1) have been described. These tumors have been passed serially for periods of 8 months to 2½ years and have maintained their original histological characteristics. One other tumor (H.S. #1), not yet permanently established in passage, has also been described. The pH range and sensitivity of the tumor to heat and ultraviolet have been recorded.