Nucleic Acid Extraction, Amplification, and Detection on Si-Based Microfluidic Platforms

Abstract

This paper gives a brief overview of silicon-based microfluidic platforms developed over the years by our group for the extraction, amplification, and detection of nucleic acids. Extraction of both genomic and viral nucleic acids from whole blood has been demonstrated on Si-based microfluidics. Rapid amplification has been achieved by polymerase chain reaction in Si-based thermal reactors (micro-PCR). Detection has been realized by a chip-to-chip integration of the micro-PCR and micro capillary electrophoresis (micro-CE).