A method for the extraction of glycogen and metabolites from a single muscle sample

Abstract

Summary An enzymatic glycogen determination is used for bovine and porcine skeletal muscle. Perchloric acid homogenates of muscle are sampled, and mixed with amyloglucosidase which extracts the glycogen and reduces it to glucose. The glucose is then analysed with specific enzymes. The use of perchloric acid homogenates allows the determination of metabolites in the protein free extract of the same homogenate. Compared with the KOH‐ethanol glycogen extraction method the amyloglucosidase method indicated higher levels of glycogen which were attributed to protein‐bound glycogen and ethanol soluble carbohydrate not determined by the KOH‐ethanol method.