Preparation and characterization of a disulfide-stabilized Fv fragment of the anti-Tac antibody: Comparison with its single-chain analog

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Ulrich K. Laemmli · Nature · 1970 · 251.6K citations

Life Sciences, Microbiology, Molecular Biology +14

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

F. William Studier, Barbara A. Moffatt · Journal of Molecular Biology · 1986 · 6.4K citations

Life Sciences, Microbiology, Molecular Biology +9

The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Application to the radioimmunoassay

A.E. Bolton, W. M. Hunter · Biochemical Journal · 1973 · 3.3K citations · Full text

Life Sciences, Biochemistry, Endocrinology +12

Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli.

James S. Huston, Dennis J. Levinson, M Mudgett-Hunter et al. · Proceedings of the National Academy of Sciences · 1988 · 1.6K citations · Full text

Engineering, Biochemistry, Molecular Biology +17

Single-Chain Antigen-Binding Proteins

Robert E. Bird, Karl D. Hardman, James W. Jacobson et al. · Science · 1988 · 1.5K citations

Pharmacy, Biochemistry, Molecular Biology +13