Time-resolved Fluoroimmunoassay in the Detection of Plant Viruses

Abstract

Summary Time-resolved fluoroimmunoassay (TR-FIA) was used to detect potato virus Y in potato leaf extracts diluted at least 106-fold, and in extracts of secondarily infected dormant tubers diluted 10- or 102-fold depending on the virus strain used. TR-FIA was also used to detect potato virus X in extracts of secondarily infected tubers diluted up to 106-fold. The response in the TR-FIA using a two-incubation method was linearly related to the virus concentration in a series of dilutions and the method can therefore be used for quantitative analysis. TR-FIA was found to be five to 100 times more sensitive than conventional double antibody sandwich ELISA.