In vivo analysis of overlapping transcription units in the rplKAJLrpoBC ribosomal protein—RNA polymerase gene cluster of Escherichia coli

Rapid and efficient site-specific mutagenesis without phenotypic selection.

Thomas A. Kunkel · Proceedings of the National Academy of Sciences · 1985 · 7.3K citations · Full text

Life Sciences, Biotechnology, Molecular Biology +15

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

Thomas A. Kunkel, John D. Roberts, Richard A. Zakour · Methods in enzymology on CD-ROM/Methods in enzymology · 1987 · 5.3K citations

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A rapid boiling method for the preparation of bacterial plasmids

David S. Holmes, Michael E. Quigley · Analytical Biochemistry · 1981 · 3.3K citations

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[32] Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors

Mark J. Zoller, Michael J. Smith · Methods in enzymology on CD-ROM/Methods in enzymology · 1983 · 1.1K citations

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DNA replication and the division cycle in Escherichia coli

D.J. Clark, O. Maaløe · Journal of Molecular Biology · 1967 · 970 citations

Life Sciences, Microbiology, Molecular Biology +16