A Screening System for the Directed Evolution of Epoxygenases: Importance of Position 184 in P450 BM3 for Stereoselective Styrene Epoxidation

Abstract

Positively charged: A screening system for the directed evolution of epoxygenases has been developed. Validation reveals that introducing a positively charged amino acid at position 184 (see picture) results in inversion of enantioselectivity in the epoxidation of styrene. The synthetic potential of epoxygenases can now be tailored by methods of directed evolution. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2006/z600255_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.