Mapping of the Grh 1 Locus for Green Rice Leafhopper Resistance in Rice Using RFLP Markers.

Abstract

We determined the chromosomal location of a green rice leafhopper(GRLH) resistance gene, Grh1, using restriction fragment length polymorphism (RFLP) markers. First, we determined the graphical genotype of Norin-PL2, to which Grh1 was introgressed from an indica variety, Pe-bi-hun, and identified four Pe-bi-hun-derived chronrosonral segments on chromosomes 5, 11 and 12. Then, we examined the linkages between Grh1 and RFLP markers located in the four introgressed segments using 108 B1F1 plants. The results indicated that the resistance gene Grh1 was located on chromosome 5 and linked to RFLP markers R566, C309, R2558, XNpb260.