Linkage map of restriction fragment length polymorphism loci in rice.

Abstract

We have constructed a genetic map of the nuclear genome in rice (Oryza sativa L.), utilizing 322 restriction fragment length polymorphism (RFLP) DNA markers and nine morphologlcal and biochemical genetlc markers. The RFLP map is based on the segregation of 144 F2 plants from a cross between Indica and Japonica varieties. The map covers a total of 1, 836 cM with 347 loci and exceeds the data recorded in the classical genetic map of rice by 58.5% and the current RFLP map (McCOUCH et al. 1988) by 32.2%. The RFLP linkage groups were assigned to the twelve chromosomes of rice using trisomic mutants andseveral genetic markers. This map should provide the basis for the mapping of genes controlling agronomic traits of rice.