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Stimulation of Ribosomal RNA Synthesis in Isolated Nuclei and Nucleoli by Partially Purified Preparations of SV40 T Antigen
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1978
Year
Stationary PhaseImmunologyMolecular BiologyCell ProliferationPartially Purified PreparationsCell CycleCell GrowthCell SpecializationCellular PhysiologyProtein SynthesisSerum DeprivationRna ProcessingSv40 T AntigenCell DivisionRna BiologyDna ReplicationGene ExpressionCell BiologyChromatinHuman CellDevelopmental BiologyNatural SciencesNucleic Acid BiochemistryRibosomal Rna SynthesisMedicine
Under conditions restrictive for growth, such as high cell density or serum deprivation, human diploid cells (Wiebel and Baserga 1969; Westermark 1971) and a number of established rodent cell lines (Todaro et al. 1965; Nilhausen and Green 1965; Holley and Kiernan 1968; Bürk 1970) enter a stationary phase in which most of the cells have a DNA content equivalent to that of cells in the G1 phase of the cell cycle (Wiebel and Baserga 1969; Bartholomew et al. 1976). When these resting cells, called G0 cells by some investigators (Epifanova and Terskikh 1969; Sander and Pardee 1972; Baserga et al. 1973; Smets 1973), are stimulated to proliferate, they enter DNA synthesis after a lag period that varies from 10 hours to more than 40 hours. The entrance into S is then followed by a wave of mitosis. Under similar conditions restrictive for growth, virally transformed mammalian cells do not enter...