Characterization of Human Mucin MUC17

Abstract

With increasing interest on mucins as diagnostic and therapeutic targets in cancers and other diseases, it is becoming imperative to characterize novel mucins and investigate their biological significance. Here, we present the completed coding sequence and genomic organization of the previously published partial cDNA sequence of MUC17. Rapid amplification of cDNA ends with PCR, sequences from the Human Genome databases, and in vitro transcription/translational assays were used for these analyses. The is and on in the The coding sequence of coding for and The of of the of the is in the we the of and in and with the The of is of and With increasing interest on mucins as diagnostic and therapeutic targets in cancers and other diseases, it is becoming imperative to characterize novel mucins and investigate their biological significance. Here, we present the completed coding sequence and genomic organization of the previously published partial cDNA sequence of MUC17. Rapid amplification of cDNA ends with PCR, sequences from the Human Genome databases, and in vitro transcription/translational assays were used for these analyses. The is and on in the The coding sequence of coding for and The of of the of the is in the we the of and in and with the The of is of and the of the and in is to and the mucins mucins in the and used amplification of cDNA of used amplification of cDNA of and and mucins the and the The mucins and of the The mucins the of and and to the of of the as of the and the The of to and is with the and in the the with and mucins The partial cDNA to to of the of in were on the cDNA the of the sequence of and the as cDNA with sequence to the with the the sequences were of the and the and the The partial cDNA of of of of The sequence of with the of of the and The to with and we the of the and with the organization of the is in for and is to we the and and and for of MUC17. of in with the and of and were used as of genomic genomic were from of to the the were the the of from of cDNA used to cDNA from with used to to the of the were in of of of of the and of The for for for and for The amplification and the the were on and with were the were and with the and in the the with and in of of of of and of The for for for and for with the of the for The for The amplification the and and amplification and The in of the The coding for with of as as and were with the to the The used for in vitro and the of sequence in the and The used as as the from the and from with and were in and to were with The amplification the and in and The the and of and of were the and of to the were were in with were to the were for were with to for in the and for were in and were as in as with the and of the of the sequence the of the of on the sequence to the the of the of The sequence to the the of the of of of and were and amplification of to on cDNA and of the The amplification on amplification were from to were to the of the The amplification with of of the amplification with of is the for the of the sequence the of of the and of the of from and and from with and and with The amplification in in and used as in on genomic The of the of with with of The of the with the of in the sequence of the were to from of and the and were for MUC17. the the the of the the the for in of were with the and the other were with the and The of the for the for the and for the of it to is of of the sequences the and were The sequence in and on the The sequence is on the sequence with as to in the we of the to the the of and of the and of the from the sequence and the partial cDNA sequence of the in the from to the and the The on from of in the sequence of the and amplification were with from to for the PCR, and from to The of amplification the for the were in the sequence of the the of the amplification is on the to of of the these the cDNA the of the of the of the of the with sequence of the to the of The sequences of the the of the and the sequence and to the organization of and sequence in is with and on in the is from to and and is to genomic and is from the of The in is and for of in with in and is in the The of the sequences were to the sequence of MUC17. and coding for of the sequence of the as as the and The of the to of sequence and the and used and as and The and were on from to were The in the of and of coding for the sequence and the of MUC17. the of in the the and sequences of the and the with sequence in the and The used as as the for the sequence in the the of on were the to the the to the the and the of to in to the of in from to The of the from to The the and is of of for the of MUC17. of is of the of the The of amplification on genomic in the and in the The amplification on to amplification and of the and sequence coding for the of the of the in of is in the sequence and is sequence of the of the from the for of the of of with and of the the of the the of the of and in of the The used for in vitro to the for the of the used The of the of is to the of the for is for it is the of sequence the of and and is is the of of the of and and of and organization and of is the of of the sequences of and in on the The of of the of in the of in and in the and sequence and these on The amplification were and were of the to the previously sequence of The the of from the of coding for and the The is the of the the and of were the of and in and amplification were of the amplification it as the sequence with the the other to of of The of in the as with the The sequence to and in the of in in of and in of the it in the in the in from the of the on and used as the of the were to The sequence the and for in sequence is the the of used to the used in from the and and from were on and for and with with the for MUC17. in the used as to the of the on and the used of to investigate on of from were used in of MUC17. The and the for used as is the the of the of were to and of sequence to the these The the of to the to and as for of from the and and from and as from as the of for and with with of The the of the of were The of is in is for and is the of of the for The to the of the with the of the of of the is in of the sequence to the sequence with the from and the from the for of The in and in the and The the of and the of The amplification were the and from to the the of the and and used in the assays for for the in the in and were for and it the the to the with and for the and in the for The in the and in the and the of the The the were with with and and of in and the were in in and and as of were in the and were for with and for for of as the in the to as and to in were for the were with and in the of the the interest in the of the mucins were to with to the the it is the of mucins as as the of the of the for in with mucins and their in for the of as the of and is to to the to with with and the used to is The of in the and of these and their for diagnostic and the and of the of the in the present the previously sequence to the sequence and the and the of the is and sequence sequence of sequence is in and of sequence the to these is sequence with for in the and for in the of sequence is in of and in in to the sequence as the and with on sequences of of in is sequence of The sequence is as of and and of the sequence with the and of and and the sequence the the and the sequence of is to and to other of the of the and their genomic we to as and the of for the of the we the of in the The of sequence the is the on the in the with is the in to with the of to the as in and present in the the to mucins in as is to The of mucins and to the sequence with the to the the the and the of in the and the the the the the of The of of and of to from of with the the for the of to in the and in for in and and and on the and the of from the of the and of on genomic and cDNA from the in the The amplification in used genomic amplification with as the and as and as we for with in the and the sequence as for the of The and the in the and of is of of the sequence in of and to to for with as in is in the in the for the sequence of is and as with the of the of the of the completed coding sequence for the is in and other is of in the of MUC17. The is in with the the the of for in and the of the and in is to and the mucins mucins in the and used amplification of cDNA of used amplification of cDNA of and and mucins the and the The mucins and of the The mucins the of and and to the of of the as of the and the The of to and is with the and in the the with and mucins The partial cDNA to to of the of in were on the cDNA the of the sequence of and the as cDNA with sequence to the with the the sequences were of the and the and the The partial cDNA of of of of The sequence of with the of of the and The to with and we the of the and with the organization of the is in for and is to we the and and and for of MUC17. of in with the and of and were used as of genomic genomic were from of to the the were the the of from of cDNA used to cDNA from with used to to the of the were in of of of of the and of The for for for and for The amplification and the the were on and with were the were and with the and in the the with and in of of of of and of The for for for and for with the of the for The for The amplification the and and amplification and The in of the The coding for with of as as and were with the to the The used for in vitro and the of sequence in the and The used as as the from the and from with and were in and to were with The amplification the and in and The the and of and of were the and of to the were were in with were to the were for were with to for in the and for were in and were as in as with the and of and were used as of genomic genomic were from of to the the were the the of from of cDNA used to cDNA from with used to to the of the were in of of of of the and of The for for for and for The amplification and the the were on and with were the were and with the and in the the with and in of of of of and of The for for for and for with the of the for The for The amplification the and and amplification and The in of the The coding for with of as as and were with the to the The used for in vitro and the of sequence in the and The used as as the from the and from with and were in and to were with The amplification the and in and The the and of and of were the and of to the were were in with were to the were for were with to for in the and for were in and were as in as with the and of the of the sequence the of the of on the sequence to the the of the of The sequence to the the of the of of of and were and amplification of to on cDNA and of the The amplification on amplification were from to were to the of the The amplification with of of the amplification with of is the for the of the sequence the of of the and of the of from and and from with and and with The amplification in in and used as in on genomic The of the of with with of The of the with the of in the sequence of the were to from of and the and were for MUC17. the the the of the the the for in of were with the and the other were with the and The of the for the for the and for the of it to is of of the sequences the and were The sequence in and on the The sequence is on the sequence with as to in the we of the to the the of and of the and of the from the sequence and the partial cDNA sequence of the in the from to the and the The on from of in the sequence of the and amplification were with from to for the PCR, and from to The of amplification the for the were in the sequence of the the of the amplification is on the to of of the these the cDNA the of the of the of the of the with sequence of the to the of The sequences of the the of the and the sequence and to the organization of and sequence in of sequence and the and used and as and The and were on from to were The in the of and of coding for the sequence and the of MUC17. the of in the the and sequences of the and the with sequence in the and The used as as the for the sequence in the the of on were the to the the to the the and the of to in to the of in from to The of the from to The the and is of of for the of MUC17. of is of the of the The of amplification on genomic in the and in the The amplification on to amplification and of the and sequence coding for the of the of the in of is in the sequence and is sequence of the of the from the for of the of of with and of the the of the the of the of and in of the The used for in vitro to the for the of the used The of the of is to the of the for is for it is the of sequence the of and and is is the of of the of and and of and organization and of is the of of the sequences of and in on the The of of the of in the of in and in the and sequence and these on The amplification were and were of the to the previously sequence of The the of from the of coding for and the The is the of the the and of were the of and in and amplification were of the amplification it as the sequence with the the other to of of The of in the as with the The sequence to and in the of in in of and in of the it in the in the in from the of the on and used as the of the were to The sequence the and for in sequence is the the of used to the used in from the and and from were on and for and with with the for MUC17. in the used as to the of the on and the used of to investigate on of from were used in of MUC17. The and the for used as is the the of the of were to and of sequence to the these The the of to the to and as for of from the and and from and as from as the of for and with with of The the of the of were The of is in is for and is the of of the for The to the of the with the of the of of the is in of the sequence to the sequence with the from and the from the for of The in and in the and The the of and the of The amplification were the and from to the the of the and and used in the assays for for the in the in and were for and it the the to the with and for the and in the for The in the and in the and the of the The the were with with and and of in and the were in in and and as of were in the and were for with and for for of as the in the to as and to in were for the were with and in the of the and of the of the sequence the of the of on the sequence to the the of the of The sequence to the the of the of of The of and were and amplification of to on cDNA and of the The amplification on amplification were from to were to the of the The amplification with of of the amplification with of is the for the of the sequence the of of the and of the of from and and from with and and with The amplification in in and used as in on genomic The of the of with with of The of the with the of in the sequence of the were to from of and the and were for MUC17. the the the of the the the for in of were with the and the other were with the and The of the for the for the and for the The of it to is of of the sequences the and were The sequence in and on the The sequence is on the sequence with as to in the we of the to the the of and of the and of the from the sequence and the partial cDNA sequence of the in the from to the and the The on from of in the sequence of the and amplification were with from to for the PCR, and from to The of amplification the for the were in the sequence of the the of the amplification is on the to of of the these the cDNA the of the of the of the of the with sequence of the to the of The sequences of the the of the and the sequence and to the organization of and sequence in the of to in to the of in from to The of the from to The the and is of of for the of MUC17. of is of the of the The of amplification on genomic in the and in the The amplification on to amplification and of the and sequence coding for the of is in the sequence and is sequence of the of the from the for of the of of with and of the the of the the of the of and in of the The used for in vitro to the for the of the used The of the of is to the of the for is for it is the of sequence the of and and is is the of of the of and and of and organization and of is the of of the of in the of in and in the and sequence and these on The amplification were and were of the to the previously sequence of The the of from the of coding for and the The is the of the the and of were the of and in and amplification were of the amplification it as the sequence with the the other to of of The of in the as with the The sequence to and in the of in in of and in of the it in the in the is the the of the of were to and of sequence to the these The the of to the to and as for of from the and and from and as from as the of for and with with of The the of the of were The of is in is for and is the of of the for The to the of the with the of the of of the is in of the sequence to the sequence with the from and the from the for of The in and in the and The the of and the of The amplification were the and from to the the of the and and used in the assays for for the in the in and were for and it the the to the with and for the and in the for The in the and in the and the of the The the were with with and the interest in the of the mucins were to with to the the it is the of mucins as as the of the of the for in with mucins and their in for the of as the of and is to to the to with with and the used to is The of in the and of these and their for diagnostic and the and of the of the in the present the previously sequence to the sequence and the and the of the is and sequence sequence of sequence is in and of sequence the to these is sequence with for in the and for in the of sequence is in of and in in to the sequence as the and with on sequences of of in is sequence of The sequence is as of and and of the sequence with the and of and and the sequence the the and the sequence of is to and to other of the of the and their genomic we to as and the of for the of the we the of in the The of sequence the is the on the in the with is the in to with the of to the as in and present in the the to mucins in as is to The of mucins and to the sequence with the to the the the and the of in the and the the the the the of The of of and of to from of with the the for the of to in the and in for in and and and on the and the of from the of the and of on genomic and cDNA from the in the The amplification in used genomic amplification with as the and as and as we for with in the and the sequence as for the of The and the in the and of is of of the sequence in of and to to for with as in is in the in the for the sequence of is and as with the of the of the of the completed coding sequence for the is in and other is of in the of MUC17. The is in with the the the of for in and the interest in the of the mucins were to with to the the it is the of mucins as as the of the of the for in with mucins and their in for the of as the of and is to to the to with with and the used to is The of in the and of these and their for diagnostic and the and of the of the in the present the previously sequence to the sequence and the and the of the is and sequence sequence of sequence is in and of sequence the to these is sequence with for in the and for in the of sequence is in of and in in to the sequence as the and with on sequences of of in is sequence of The sequence is as of and and of the sequence with the and of and and the sequence the the and the sequence of is to and to other of the of the and their genomic we to as and the of for the of the we the of in the The of sequence the is the on the in the with is the in to with the of to the as in and present in the the to mucins in as is to The of mucins and to the sequence with the to the the the and the of in the and the the the the the of The of of and of to from of with the the for the of to in the and in for in and and and on the and the of from the of the and of on genomic and cDNA from the in the The amplification in used genomic amplification with as the and as and as we for with in the and the sequence as for the of The and the in the and of is of of the sequence in of and to to for with as in is in the in the for the sequence of is and as with the of the of the of the completed coding sequence for the is in and other is of in the of MUC17. The is in with the the the of for in and and for and for the the of the of for The Human is for the for