FEBS Letters · 1990 · 299 citations · 20 references
Life SciencesMolecular BiologyPharmacologyCell SignalingNeuropharmacologySignal TransductionCell Signaling NetworksG Protein-coupled Receptor SignalingHistamine ReleaseMast Cell ActivationNeurotransmissionSmall Gtpase SignalingG-protein-coupled Receptor Signaling In SecretionHistamine ReceptorsMast Cell ExocytosisMast Cell Signaling PathwaysNeuropeptide AgonistsNeuropeptide SecretionNeuropeptide-induced ActivationSubstance P Pharmacology
The neuropeptide substance P and the polyamine compound 48/80, both known to activate mast cell secretory processes, increased the rate of GTP S binding to G-proteins purified from calf brain (Go/Gi mixture). The GTPase activity of G-proteins was also increased by substance P and compound 48/80 in a dose-dependent and Mg2+-dependent way. These effects were similar to those of the wasp venom peptide mastoparan, another histamine releaser of rat peritoneal and human skin mast cells. This suggests that the secretory property of compound 48/80 and substance P is not due to a receptor-mediated process but, like mastoparan, results from a direct activation of G-proteins.
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