The High Pressure Liquid Chromatogrpaphy of Metal Chelates of Diethyldithiocarbmic Acid and Dithizone

Abstract

Abstract The HPLC behavior of Ni(II), Co(III), Cu(II), Zn(II), Cd(II), Hg(II) and Pb(II) chelates of diethyldithiocarbamic acid, H(DDTC), and dithizone, H(Dz), was studied on silica gel columns with toluene as the mobile phase. Calibration plots gave linear results for plots of peak area vs. amount of metal injected over the low nanogram to milligramrange. Calibration plots for the above cations were determined on the basis of 3 to 5 replicate determinations for 8 to 10 different concentration levels over the above range with a relative standard deviation less than 5.0% for all metals studied. A variable wavelength photometric detector was used and the detection limits at 270 nm were in the range of 5 to 200 ng depending on the metal species. The best separations were obtained on a1/4″ × 35 cm stainless-steel packed with 10 micron μ-Porasil.