Control of 4E‐BP1 expression in mouse brown adipose tissue by the β₃‐adrenoceptor

Abstract

Knockout of the translation inhibitor 4E‐BP1 induces an overexpression of uncoupling protein‐1 (UCP1) [Nature Medicine 7 (2001) 1128]. A possible inverse control of UCP1 and 4E‐BP1 expressions in mouse brown adipose tissue was investigated. Cold‐exposure, which increases the expression of UCP1, decreased that of 4E‐BP1 mRNA in wild type but not in β 1 /β 2 /β 3 ‐adrenoceptor knockout mice. Administration of the β 3 ‐adrenoceptor agonist CL 316 246 decreased 4E‐BP1 mRNA by 75% and protein by 41% after 6 and 48 h, respectively. Our data are the first report of a regulation by the β 3 ‐adrenoceptor of 4E‐BP1 expression. They support a role of the latter in adaptive thermogenesis.