Functional analysis of the C‐terminal region of the vacuolar cadmium‐transporting rice OsHMA3

Abstract

Rice OsHMA3 is a vacuolar cadmium (Cd) transporter belonging to the P 1B ‐ATPase family and has a long (273aa) C‐terminal region. We analyzed the function of the region related to Cd using the transgenic Arabidopsis Col‐0 ecotype, which is sensitive to Cd. The OsHMA3 variant containing a truncated (58aa) C‐terminal region did not confer Cd tolerance, whereas an OsHMA3 variant containing a longer truncated (105aa) C‐terminal region conferred Cd tolerance to transgenic Arabidopsis . We conclude that the C‐terminal region, particularly the region containing the first 105aa, has an important role in OsHMA3 activity.