Detection of Hb-Papio B, a Silent Mutation of the Baboon β Chain, by High Performance Liquid Chromatography. Improved Procedures for the Separation of Globin Chains by Hplc

Abstract

A silent mutant of the β chain of the baboon (Papio cynocephalus) was detected by high performance liquid chromatography of the globin chains. The substitution is β13 Ala → Thr. The inclusion of nonylamine in the developers for HPLC results not only in an improved separation of baboon globin chains but also in the complete resolution of the human Gγ, Aγ, and AγT chains and some common human variant β chains.