Myelinated Axons Demonstrated in the CNS and PNS by Anti‐Neurofilament Immunoreactivity and Luxol Fast Blue Counterstaining

Abstract

A method which demonstrates myelinated axons in the central and peripheral nervous systems will be described. Paraffin, frozen or semi-thin epoxy-embedded sections were immunostained first with a monoclonal antibody raised against a 200 kilo-Dalton neurofilament protein and then counter-stained with Luxol fast blue.