The Proteolytic Enzyme Test for Detecting Incomplete Antibodies

Abstract

The specific agglutination by incomplete anti D antibodies of a saline suspension of red cells, after preliminary incubation with a solution of crystalline trypsin, has been previously reported (Morton and Pickles, 1947). During the past two years this method has been standardized and used as a test for the detection of incomplete antibodies. The trypsin-treated cells have been found to give reliable results and to be more sensitive than the concentrated albumin (Diamond and Denton, 1945) and anti-globulin (Coombs, Mourant, and Race, 1945) methods in the detection of weak incomplete anti D antibodies. The utilization of trypsin-treated red cells for the routine detection of anti Rh antibodies has been reported by