Differential Protein Expression Profiles of Gastric Epithelial Cells Following <i>Helicobacter </i><i>p</i><i>ylori </i>Infection Using ProteinChips

Abstract

Helicobacter pylori infects approximately half of the world's population and the bacterium is associated with gastric cancer and peptic and duodenal ulcers. In this study, Surface Enhanced Laser Desorption /Ionization time-of-flight mass spectrometry (SELDI-TOF-MS) was used to identify the biomarkers from H. pylori infected gastric epithelial cells (GEC) to understand key mechanisms associated with pathogenesis. Using different chip surfaces, differential protein expression profile of GEC was obtained and several upregulated or downregulated biomarkers were detected on GEC, following H. pylori infection. Four different H. pylori infected GECs were compared based on their expression of MHC class II, a receptor reported to trigger apoptosis. One biomarker was identified in H. pylori infected GEC as Annexin A2 (Annexin II) from the flow through of the anion-exchange resin. The increased expression of Annexin II in GEC following H. pylori infection was further confirmed by Western Blot analyses and indicates its involvement in H. pylori pathogenesis. Keywords: Helicobacter pylori • gastric epithelial cell • pathogenesis • surface enhanced laser desorption/ionization time off flight mass spectrometry (SELDI-TOF−MS) • ProteinChips • differential protein expression profile • biomarker • annexin II • gastric carcinoma