Angewandte Chemie International Edition · 1998 · 25 citations · 0 references
The simultaneous acquisition of in-phase and antiphase multiplets with high sensitivity and minimum overlap (see section of 2D spectra on the right) is possible in a novel NMR experiment. Based on this method, homonuclear coupling constants such as the <sup>3</sup> J(H<sup>N</sup> ,H<sup>α</sup> ) couplings in peptides and proteins can be determined quantitatively without isotope labeling.