The T7 phage gene 10 leader RNA, a ribosome-binding site that dramatically enhances the expression of foreign genes in Escherichia coli

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Ulrich K. Laemmli · Nature · 1970 · 251.6K citations

Life Sciences, Microbiology, Molecular Biology +14

High resolution two-dimensional electrophoresis of proteins.

Patrick H. O’Farrell · Journal of Biological Chemistry · 1975 · 19.4K citations · Full text

Life Sciences, Biochemistry, Bioseparations +13

Rapid and efficient site-specific mutagenesis without phenotypic selection.

Thomas A. Kunkel · Proceedings of the National Academy of Sciences · 1985 · 7.3K citations · Full text

Life Sciences, Biotechnology, Molecular Biology +15

The 3′-Terminal Sequence of <i>Escherichia coli</i> 16S Ribosomal RNA: Complementarity to Nonsense Triplets and Ribosome Binding Sites

John Shine, L. Dalgarno · Proceedings of the National Academy of Sciences · 1974 · 3.6K citations · Full text

Life Sciences, Microbiology, Molecular Biology +14

An improved assay for nanomole amounts of inorganic phosphate

Peter A. Lanzetta, Lawrence J. Alvarez, Peter S. Reinach et al. · Analytical Biochemistry · 1979 · 2.2K citations

Natural Sciences, Biochemistry, Chemistry +6