FEBS Letters · 1984 · 118 citations · 8 references
Life SciencesBiochemistryMicrobiologyCarbohydrate BiochemistryEnzymologyMicrobial MetabolismEnzyme InhibitionEnzyme KineticsFungal Natural Product BiosynthesisGlycan BiosynthesisGlycan DegradationGlycosidic Bond HydrolysisBeta-glucanase TreatmentCell Wall Polysaccharide SynthesisCell Wall Synthesis InhibitionFungal Glycan BiosynthesisGlycosidase InhibitionOligosaccharide ProcessingPeptidoglycan Biosynthesisβ-Glucosidase Activity
The cyclic peptide antibiotic echinocandin was found to inhibit 1,3-beta-D-glucan synthase activity present in a mixed membrane fraction from Candida albicans. Addition of antibiotic reduced the Vmax of the enzyme, but the Km was unaffected. GTP stimulated enzyme activity approximately 4-fold, but did not affect the percentage inhibition of the enzyme by echinocandin. Treatment of the reaction products with alpha-amylase and beta-glucanase confirmed that the polymer synthesised was 1,3-beta-D-glucan, not glycogen.
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F. Benz, F. Knüsel, J. Nüesch et al. · Helvetica Chimica Acta · 1974 · 249 citations
Papulacandin B: an Inhibitor of Glucan Synthesis in Yeast Spheroplasts
Bruce C. Baguley, GÜNTER RÖMMELE, J. Gruner et al. · European Journal of Biochemistry · 1979 · 135 citations · Full text