Journal of Proteome Research · 2008 · 20 citations · 21 references
Life SciencesBiochemistryOncologyGlycobiologyMolecular OncologyProteomicsCancer GlycobiologyGlycan AnalysisGlycoproteomicsGlycosylation PathwaysO-linked GlycosylationProtein GlycosylationAberrant Glycosylation In CancerAberrant O-glycosylation In CancerCleavageGlycan Structure AnalysisGlycomics Of CancerGlycosylation In DiseaseO-glycan AnalysisTumor-associated Glycoproteins
Recent investigations have implicated aberrant glycosylations in various malignancies, including epithelial ovarian cancer (EOC). The protocol here identifies O-linked carbohydrate patterns in EOC plasma glycoproteins through chemical cleavage and purification of these glycans. Dialyzed plasma is subjected to reductive beta-elimination with alkaline borohydride to release O-linked oligosaccharides from glycoproteins. Enrichment of released glycans, as well as removal of peptide and other contaminants, is followed by carbohydrate pattern analysis with MALDI-FT-ICR-MS.
21